Plasmid-encoded protein attenuates Escherichia coli swimming velocity and cell growth,not reprogrammed regulatory functions |
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Authors: | Chelsea Virgile Pricila Hauk Hsuan-Chen Wu William E. Bentley |
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Affiliation: | 1. Institute for Bioscience and Biotechnology Research, MD, 20742 Fischell Dept. of Bioengineering, University of Maryland, MD, 20742;2. Dept. of Biochemical Science and Technology, National Taiwan University, Taipei City, Taiwan;3. Institute for Bioscience and Biotechnology Research, MD, 20742 |
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Abstract: | In addition to engineering new pathways for synthesis, synthetic biologists rewire cells to carry out “programmable” functions, an example being the creation of wound-healing probiotics. Engineering regulatory circuits and synthetic machinery, however, can be deleterious to cell function, particularly if the “metabolic burden” is significant. Here, a synthetic regulatory circuit previously constructed to direct Escherichia coli to swim toward hydrogen peroxide, a signal of wound generation, was shown to work even with coexpression of antibiotic resistance genes and genes associated with lactose utilization. We found, however, that cotransformation with a second vector constitutively expressing GFP (as a marker) and additionally conferring resistance to kanamycin and tetracycline resulted in slower velocity (Δ~6 μm/s) and dramatically reduced growth rate (Δ > 50%). The additional vector did not, however, alter the run-and-tumble ratio or directional characteristics of H2O2–dependent motility. The main impact of this additional burden was limited to slowing cell velocity and growth, suggesting that reprogrammed cell motility by minimally altering native regulatory circuits can be maintained even when extraneous burden is placed on the host cell. © 2019 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2778, 2019. |
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Keywords: | CheZ metabolic burden constitutive fluorescence motility synthetic biology |
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