Metabolic engineering to improve 1,5-diaminopentane production from cellobiose using β-glucosidase-secreting Corynebacterium glutamicum |
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| Authors: | Rena Matsuura Mayumi Kishida Rie Konishi Yuuki Hirata Noriko Adachi Shota Segawa Kenta Imao Tsutomu Tanaka Akihiko Kondo |
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| Institution: | 1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan;2. Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan |
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| Abstract: | Microbial production of 1,5-diaminopentane (DAP) from renewable feedstock is a promising and sustainable approach for the production of polyamides. In this study, we constructed a β-glucosidase (BGL)-secreting Corynebacterium glutamicum and successfully used this strain to produce DAP from cellobiose and glucose. First, C. glutamicum was metabolically engineered to produce l -lysine (a direct precursor of DAP), followed by the coexpression of l -lysine decarboxylase and BGL derived from Escherichia coli and Thermobifida fusca YX (Tfu0937), respectively. This new engineered C. glutamicum strain produced 27 g/L of DAP from cellobiose in CGXII minimal medium using fed-batch cultivation. The yield of DAP was 0.43 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose), which is the highest yield reported to date. These results demonstrate the feasibility of DAP production from cellobiose or cellooligosaccharides using an engineered C. glutamicum strain. |
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| Keywords: | 1 5-diaminopentane β-glucosidase cellobiose Corynebacterium glutamicum metabolic engineering |
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