Differentiation in human amniotic fluid cell cultures: Chorionic gonadotropin production |
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Authors: | Robert E. Priest Jean H. Priest Jessie F. Moinuddin Demetrios S. Sgoutas |
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Affiliation: | (1) Department of Pathology and Laboratory Medicine, Emory University, 30322 Atlanta, Georgia;(2) Department of Pediatrics, Division of Medical Genetics, Emory University, 30322 Atlanta, Georgia |
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Abstract: | Summary Two of the distinguishable cell classes subcultured from human amniotic fluid were examined for their capability to produce human chorionic gonadotropin (hCG) as determined by radioimmunoassay. The class that predominates in most cultures used for prenatal genetic diagnosis, previously termed AF (for amniotic fluid), secretes hCG into the culture medium. Dermal fibroblasts do not, nor does another type of cultured cell from amniotic fluid, previously termed F because of a resemblance to fibroblasts. Primary AF cultures produce more hCG than do subcultures. Evidence that this hormone is intact hCG is provided by its immunoreactivity with antisera raised against the β-subunit and against the intact molecule of hCG. Furthermore, a dose-response curve for hormone in culture medium is parallel to that of highly purified intact hCG. It is postulated that AF cultures are derived from fetal membranes and retain properties of trophoblast. Research supported by Grand HD 11379 from the National Institutes of Health. |
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Keywords: | human amniotic fluid cell cultures hCG (human chorionic gonadotropin) radioimmunoassay trophoblast |
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