Existence of Tenascin-C Isoforms in Rat that Contain the Alternatively Spliced AD1 Domain are Developmentally Regulated During Hippocampal Development |
| |
Authors: | J Garwood U Theocharidis V Calco A Dobbertin A Faissner |
| |
Institution: | (1) Department of Cell Morphology and Molecular Neurobiology, Ruhr University, NDEF 05/593, Universit?tsstra?e 150, 44780 Bochum, Germany;(2) D?partement Neurotransmission et S?cr?tion Neuroendocrine, Institut des Neurosciences Cellulaires et Int?gratives (UPR 3212), Centre National de la Recherche Scientifique et Universit? de Strasbourg, 5 rue Blaise Pascal, 67084 Strasbourg, France;(3) Universit? Paris Descartes, INSERM U686, Centre universitaire des Saints-P?res, 75006 Paris, France |
| |
Abstract: | Tenascin-C (TN-C) is a multimodular glycoprotein of the extracellular matrix which is important for the development of the
nervous system and has a range of different functions which are mediated by the different protein domains present. TN-C contains
eight constitutive fibronectin type III (FNIII) domains and a region of alternatively spliced FNIII domains. In the mouse
and chick, six of these domains have been described and characterized, whereas in human there are nine of them. In this report,
we show that seven alternatively spliced FNIII domains exist in rat and describe the differential expression pattern of the
additional domain AD1 during embryonic and postnatal rat brain development. The AD1 domain of rat is homologous to the ones
described in human and chick proteins but does not exist in mouse. Its expression can be located to the developing rat hippocampus
and the lining of the lateral ventricle, regions where the TN-C protein may affect the behavior of stem and progenitor cells.
During hippocampal development AD1 and the other alternatively spliced domains are differentially expressed as shown by RT-PCRs,
immunocytochemistry and in situ hybridizations. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|