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| 利用一种新型反应器——BelloCell培养动物细胞 | |
| 引用本文： | 郑云程,吴建虹,孙奋勇,廖美德,谢秋玲. 利用一种新型反应器——BelloCell培养动物细胞[J]. 生物学杂志, 2010, 27(1): 20-23. DOI: 10.3969/j.issn.1008-9632.2010.01.020 |
| 作者姓名： | 郑云程 吴建虹 孙奋勇 廖美德 谢秋玲 |
| 作者单位： | 1. 暨南大学生物工程研究所,广州,510632 2. 深圳市中药药学及分子药理学研究重点实验室,深圳,518057 3. 华南农业大学天然农药与化学生物学教育部重点实验室,广州,510642 |
| 基金项目： | 广东省生物工程药物重点实验室开放课题 |
| 摘 要： | 利用Bello Cell这种新型的生物反应器，来培养COS7细胞和昆虫Sf9细胞。COS7细胞和昆虫sf9细胞分别用T75培养瓶及spinnerflask培养之后，经细胞计数接入Bello Cell之中，同时检测并分析培养基中葡萄糖、谷氨酰胺、乳酸和氨浓度变化情况。COS7细胞初始接种量为4．208×10^7cells，最终在培养156h后细胞数量达到了4．68×10^8cells，是初始细胞量的11倍。sf9细胞初始接种细胞量为1×10^8cells，在培养192h时，细胞总量达到了最高为4．01×10^9cells，是最初细胞量的40倍。培养基的代谢物进行有规律的变化。Bello Cell适合COS7细胞和昆虫Sf9细胞高密度大规模培养，为动物细胞高效大规模表达药物蛋白，奠定重要的基础。 |
| 关 键 词： | COS7细胞 昆虫细胞 细胞培养 BelloCell反应器 |
Cultivation of animal cell in a novel bioreactor-BelloCell |
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| ZHENG Yun-cheng,WU Jian-hong,SUN Fen-yong,LIAO Mei-de,XIE Qiu-ling. Cultivation of animal cell in a novel bioreactor-BelloCell[J]. Journal of Biology, 2010, 27(1): 20-23. DOI: 10.3969/j.issn.1008-9632.2010.01.020 | |
| Authors: | ZHENG Yun-cheng WU Jian-hong SUN Fen-yong LIAO Mei-de XIE Qiu-ling |
| Affiliation: | ZHENG Yun-cheng1,WU Jian-hong2,SUN Fen-yong1,LIAO Mei-de3,XIE Qiu-ling1(1.Bioengineering Institute,Jinan University,Guangzhou 510632,2.State Key Laboratory of Chinese Medicine , Molecular Pharmacology,Shenzhen 518057,3.Key Laboratory of Pesticide & Chemical Biology Ministry of Education,South China Agricultural University,Guangzhou 510642,China) |
| Abstract: | BelloCell is a novel oscillating bioreactor,it was used for the cultivation of Cos7 cell and insect cell.After cultured in T75 flask and spinner flask with determined cell density and for cell inoculation,contens of glucose,lactate,ghtamine,and ammonia in the culture supematant were analyzed.Result showed that Cos7 cell was inoculated with 4.208×10~7 cells and reached a maximum Brimber of 4.68×10~8 cells at 156h of culture.The S f9 cell density increased from 1×10~8 cells to 4.01×10~9 cells after 192h culture.BelloCell was suitable for culturing Cos7 cell and insect cell with high density and large scale, It will play an important influence on large scale expressing drug protein with animal cell in the future. |
| Keywords: | Cos7 cell insect cell cell culture BelloCell bioreactor |
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