Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation |
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Authors: | K Hayasaka M Sato H Mitani A Shima |
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Institution: | Laboratory of Radiation Biology, Faculty of Science, University of Tokyo, Japan. |
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Abstract: | 1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells. |
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