Matrix proteins associated with bone calcification are present in human vascular smooth muscle cells grownin vitro |
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Authors: | Arlen R. Severson Ronald T. Ingram Lorraine A. Fitzpatrick |
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Affiliation: | (1) Department of Anatomy and Cell Biology, School of Medicine, University of Minnesota, 55812 Duluth, Minnesota;(2) Endocrine Research Unit, Department of Internal Medicine, Mayo Clinic and Mayo Foundation, 55905 Rochester, Minnesota;(3) Present address: Telios Pharmaceuticals Inc., 4757 Nexus Centre Drive, 92121 San Diego, CA |
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Abstract: | Summary Atherosclerotic lesions are composed of cellular elements that have migrated from the vessel lumen and wall to form the cellular component of the developing plaque. The cellular elements are influenced by various growth-regulatory molecules, cytokines, chemoattractants, and vasoregulatory molecules that regulate the synthesis of the extracellular matrix composing the plaque. Because vascular smooth muscle cells (VSMC) constitute the major cellular elements of the atherosclerotic plaque and are thought to be responsible for the extracellular matrix that becomes calcified in mature plaques, immunostaining for collagenous and noncollagenous proteins typically associated with bone matrix was conducted on VSMC grownin vitro. VSMC obtained from human aorta were grown in chambers on glass slides and immunostained for procollagen type I, bone sialoprotein, osteonectin, osteocalcin, osteopontin, decorin, and biglycan. VSMC demonstrated an intense staining for procollagen type I, and a moderately intense staining for the noncollagenous proteins, bone sialoprotein and osteonectin, two proteins closely associated with bone mineralization. Minimal immunostaining was noted for osteocalcin, osteopontin, decorin, and biglycan. The presence in VSMC of collagenous and noncollagenous proteins associated with bone mineralization suggest that the smooth muscle cells in the developing atherosclerotic plaque play an important role in the deposition of the extracellular matrix involved in calcification of developing lesions. |
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Keywords: | atherosclerosis calcification extracellular matrix proteins collagen noncollagenous proteins |
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