Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510 |
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Authors: | Zhang Zhenqing Yu Guangli Guan Huashi Zhao Xia Du Yuguo Jiang Xiaolu |
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Institution: | Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, China. |
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Abstract: | Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed. |
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Keywords: | Alginate lyase Guluronic acid Mannuronic acid Alginate Oligosaccharides |
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