Agrocinopine A, a phosphorylated opine is secreted from crown gall cells |
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Authors: | Messens E Lenaerts A Hedges R W Van Montagu M |
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Affiliation: | Rijksuniversiteit Gent, Laboratorium voor Histologie, Ledeganckstraat 35, Gent, Belgium. |
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Abstract: | We showed that phosphorus-containing metabolites of crown gall tissues were all taken up by appropriate pTi+ agrobacteria. All but one were also taken up by pTi- bacteria. This one compound, produced by nopaline-, but not by octopine-type tumours, was the only phosphorylated organic compound actively secreted by healthy crown gall cells, and it appears to be agrocinopine A. Testing crown gall cell exudates may be a general procedure for the identification of opines by transformed plant cells. |
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