天津地区寄生烟粉虱的桨角蚜小蜂分子鉴定和遗传多样性分析

挖掘本土天敌资源是害虫生物防治的有效手段。桨角蚜小蜂Eretmocerus spp.是烟粉虱Bemisia tabaci重要的寄生性天敌之一，明确桨角蚜小蜂本地种类及遗传分化关系，对本土天敌资源挖掘具有重要意义。本研究在天津5个地区采集了13个地理、寄主的桨角蚜小蜂种群，利用线粒体mtDNA COI基因片段作为分子标记，进一步通过MEGAX、DnaSP 5.10等软件进行遗传分化分析。结果表明，本研究所采种群中包含2种桨角蚜小蜂，其中测得蒙氏桨角蚜小蜂Eretmocerus mundus mtDNA COI基因序列20条（755 bq），未命名桨角蚜小蜂Eretmocerus sp. WTT-2016 mtDNA COI基因序列20条（739 bq）。两种桨角蚜小蜂的遗传多样性均较低，其中蒙氏桨角蚜小蜂Hd = 0.368，Pi = 0.00557，K = 4.205；未命名桨角蚜小蜂Hd = 0.616，Pi = 0.00106，K = 0.784。错配分析表明，蒙氏桨角蚜小蜂在天津种群较稳定，近年来未出现扩张现象。

Molecular identification and genetic diversity analysis on Bemisia tabaci parasitic wasp, Eretmocerus spp. in Tianjin

Excavating native natural enemy resources is an effective strategy in biological control of pests. Eretmocerus spp. are an important parasitic natural enemies of Bemisia tabaci. It is important to definite the relation of genetic differentiation of the local population for exploiting native natural enemy resources. In this study, 13 Eretmocerus sp.geographical and host populations were collected in 5 districts of Tianjin City. The mitochondrial mtDNA COI gene was used as a molecular marker, and MEGAX, DnaSP 5.10 software was used to carry out the genetic differentiation analysis. The results showed that there were two species of Eretmocerus among the 40 samples. There were 20 mtDNA COI gene sequences (755 bq) of Eretmocerus mundus and 20 mtDNA COI gene sequences (739 bq) of Eretmocerus sp.WTT-2016. The genetic diversity of the two species were both slight. The Hd, Pi, and K value of E. mundus were 0.368, 0.00557, and 4.205 respectively; and they were 0.616, 0.00106, 0.784 for Eretmocerus sp. respectively. Mismatch analysis showed that the population of E. mundus was stable in Tianjin, and there was no expansion in recent years.