The RNA binding protein Larp1 regulates cell division,apoptosis and cell migration |
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| Authors: | Carla Burrows Normala Abd Latip Sarah-Jane Lam Lee Carpenter Kirsty Sawicka George Tzolovsky Hani Gabra Martin Bushell David M. Glover Anne E. Willis Sarah P. Blagden |
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| Affiliation: | 1.Department of Molecular Oncology, Imperial College, Hammersmith Campus, Du Cane Road, London W12 0HS, 2.NHS Blood and Transplant, John Radcliffe Hospital, Oxford, OX3 9BQ, 3.School of Pharmacy, Nottingham University, Nottingham UK NG7 2RD and 4.Department of Genetics, Downing Site, Cambridge CB2 3EH, UK |
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| Abstract: | The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration. |
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