Functional Diversity in Fungal Fatty Acid Synthesis: THE FIRST ACETYLENASE FROM THE PACIFIC GOLDEN CHANTERELLE,CANTHARELLUS FORMOSUS* |
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Authors: | Brenda J. Blacklock Brian E. Scheffler Michael R. Shepard Naomi Jayasuriya Robert E. Minto |
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Affiliation: | From the ‡Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana 46202 and ;the §Genomics and Bioinformatics Research Unit, Jamie Whitten Delta States Research Center, Stoneville, Mississippi 38776 |
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Abstract: | Acetylenic specialized metabolites containing one or more carbon-carbon triple bonds are widespread, being found in fungi, vascular and lower plants, marine sponges and algae, and insects. Plants, moss, and most recently, insects, have been shown to employ an energetically difficult, sequential dehydrogenation mechanism for acetylenic bond formation. Here, we describe the cloning and heterologous expression in yeast of a linoleoyl 12-desaturase (acetylenase) and a bifunctional desaturase with Δ12-/Δ14-regiospecificity from the Pacific golden chanterelle. The acetylenase gene, which is the first identified from a fungus, is phylogenetically distinct from known plant and fungal desaturases. Together, the bifunctional desaturase and the acetylenase provide the enzymatic activities required to drive oleate through linoleate to crepenynate and the conjugated enyne (14Z)-dehydrocrepenynate, the branchpoint precursors to a major class of acetylenic natural products. |
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Keywords: | Enzymes Fatty Acid Fatty Acid Metabolism Fungi Lipid Synthesis Acetylenase Basidiomycetes Desaturase Polyacetylene Secondary Metabolism |
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