兔VX2肺外周肿瘤超声造影特征与CD31和CD34微血管密度的相关性研究

摘要 目的：探讨兔VX2肺外周肿瘤超声造影特征与CD31和CD34微血管密度的相关性研究。方法：15只雌性新西兰白兔进行VX2 肺肿瘤模型,总共有30个VX2 癌植入15只兔子的肺中。通过高倍镜分析不同期VX2肿瘤的MVD。通过双重免疫荧光化学染色分析不同生长期VX2肿瘤Ki67和CD31阳性表达。分析肿瘤形态学组织病理学结果与微血管分布和CEUS结果之间的相关性。结果：随着生长周期的进展,VX2肿瘤的MVD增大（P<0.05）。随着生长周期的进展,VX2肿瘤内Ki67和CD31的阳性表达率增加（P<0.05）。CEUS显示VX2 肿瘤在早期动脉期有明显的外周增强和短暂的内部增强。肺外周VX2 肿瘤中 PI和CD31 MVD值之间呈显著正相关性（r=0.734,P<0.05）。CD31 MVD和CD34 MVD之间呈负相关（r=-0.691,P<0.05）。PI和CD34 MVD值之间不存在显著相关性（r=-0.412,P>0.05）。结论：与CD34 MVD 相比,CD31 MVD 可以更有效地评估肿瘤血管生成。CEUS作为一种无创成像方法,可以有效评估兔周围型肺癌的肿瘤血管生成情况。

Correlation between Rabbit VX2 Pulmonary Outer Tumors Ultrasound Contrast Characteristics and CD31 and CD34 Microvascular Density

ABSTRACT Objective: To investigate the correlation between rabbit VX2 pulmonary outer peripheral tumors and CD31 and CD34 microvascular density. Methods: Fifteen female New Zealand white rabbits were subjected to the VX2 lung tumor model, and a total of 30 VX2 carcinomas were implanted in the lungs of 15 rabbits. The MVD of different stages of VX2 tumors was analyzed by high magnification. The positive expression of Ki67 and CD31 in VX2 tumors at different growth stages was analyzed by double immunofluorescence chemical staining. The correlations between tumor morphology and histopathology results and microvascular distribution and CEUS results were analyzed. Results: As the growth cycle progressed, the MVD of Vx2 tumors increased (P<0.05). As the growth cycle progresses, the positive expression rate of Ki67 and CD31 in Vx2 tumors increased (P<0.05). CEUs showing VX2 tumors in the early artery period. There is a significant peripheral enhancement and short interior. There''s significant positive correlation between PI and CD31 MVD values in peripheral VX2 tumors of the lung(R=0.734, P<0.05). The CD31 MVD and the CD34 MVD were negatively correlated (R=-0.691, P<0.05). There was no significant correlation between PI and CD34 MVD values(R=-0.412, P>0.05). Conclusion: CD31 MVD can be more effectively assessing tumor angiogenesis more effectively compared to CD34 MVD. As a non-invasive imaging method, CEUS can effectively assess tumor angiogenesis of rabbit surrounding lung cancer.