A potassium transport mutant of Saccharomyces cerevisiae |
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Authors: | José Ramos Pilar Contreras Alonso Rodríguez-Navarro |
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Affiliation: | (1) Departamento de Microbiologia, Escuela Técnica Superior de Ingenieros Agrónomos, E-14071 Córdoba, Spain |
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Abstract: | A mutant in Saccharomyces cerevisiae required one hundred times more K+ than wild type for the same half maximal growth rate. Mutant cells and wild type cells grown at millimolar K+ did not show significant differences in Rb+ transport. In the mutant, a rapid K+ loss induced by azide or incubation (4 h) in K+-free medium decreased the Rb+ transport Km by one half; in the wild type, those treatments decreased the Rb+Km twenty and one hundred times, respectively. Mutant and wild type did not show significant differences in Na+ transport and in the Na+ inhibition of Rb+ transport, either in normal-K+ cells or in K+-starved cells. The results suggest that either two systems or one system with two interacting sites mediate K+ transport in S. cerevisiae.Abbreviations YPD yeast-peptone-dextrose medium |
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Keywords: | Saccharomyces cerevisiae Potassium transport mutant Rubidium transport Sodium transport |
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