Actin in Mucor rouxii |
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Authors: | Myrna Sabanero Roberto Zazueta |
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Institution: | Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universida de Guanajuato, Guanajuato, Mexico |
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Abstract: | Abstract Rhodamine-conjugated phalloidin was used to analyze the actin distribution during hyphal formation in Mucor rouxii . The occurrence of actin patches in the cortical region of the cells was seen in the initial stages of growth. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-sepharose. This peptide was identified on immunoblots when polyclonal antibodies against rabbit muscle actin were used as a probe. These results indicate: (1) that changes in actin localization accompany the hyphal development and (2) the fungal 43 kDa protein shares properties that are common to muscle actin. |
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Keywords: | Actin Mucor rouxii Hyphal formation |
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