Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae |
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| Authors: | Tomonari Hirano Toshinari Godo Kazumitsu Miyoshi Keiko Ishikawa Masaya Ishikawa Masahiro Mii |
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| Institution: | (1) Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University, 648 Matsudo, Matsudo Chiba, 271-8510, Japan;(2) Botanic Gardens of Toyama, 42 Kamikutsuwada, Fuchu-machi, Nei-gun, Toyama 939-2713, Japan;(3) Crop Science Laboratory, Faculty of Bioresources, Akita Prefectural University, 241-438 Shimoshinjo Nakano, Kaidohata-Nishi, Akita 010-0195, Japan;(4) Japan Horticultural Production and Research Institute, 2-5-1 Kamishiki, Matsudo Chiba, 270-2221, Japan;(5) Environmental Stress Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba Ibaraki, 305-8602, Japan;(6) Present address: Division of Innovative Research, Creative Research Initiative “Sousei” (CRIS), Hokkaido University, Kita21, Nishi10, Kita-ku, Sapporo 001-0021, Japan |
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| Abstract: | In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at 4°C, showed no decrease
in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease
in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method
for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation
by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions. |
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| Keywords: | Cryopreservation Orchid Phaius tankervilleae Seed storage Vitrification |
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