用巨细胞病毒抗原致敏的冻干血球作间接血凝试验

本文报告用巨细胞病毒(CMV)抗原致敏的冻干绵羊红细胞(简称冻干血球)做间接血凝试验。用5％正常兔血清和10％蔗糖磷酸盐缓冲液作保护剂,将巨细胞病毒抗原致敏的绵羊红细胞进行真空冷冻干燥,制成了冻干血球。经反复检测发现,用含百万分之一Tween20、2％正常兔血清的磷酸缓冲液稀释冻干血球,可消除冻干过程中产生的非特异性凝集反应。所建立的方法重复性较好,特异性与ELISA相同,敏感性比CF高。

DETECTION QF CMV ANTIBODY USINGF REEZE-DRIED ERYTHROCYTES BYAN INDIRECT HEMAGGLUTINATION TEST

This paper reported a study on the methodology for the detection of cytcmegalcvirus antibody using freeze-dried CMV antigen-sensitized sheepREC ( SRBC ) by an indirect hemagglutination test. Freeze-drying of CMV antigen-sensitized SRBC was carried out in PBS ( pH 7.0) contaning 5%heat-inactivated normai rabbit serum and 10% sucrose. The main obstacle tothe use of such SRBC was a nonspecific agglutination occu.rring after lyop-hilization of CMV-sensitized SRBC. This obstacle can be overcome by ad-ding Tween 20 ( 1/1 000 000 ) to the phosphate-buffered saline in which theCMV-sensitized SRBC were resuspended for use.The method we established has fairly good reproducibility. The specifi-city of the method was the same as ELISA and the sensitivity was higherthan the CF.