Fermentation pattern of Zymomonas mobilis at high sucrose concentrations |
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Authors: | Horst W. Doelle Paul F. Greenfield |
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Affiliation: | (1) Biotechnology Unit, Department of Microbiology, University of Queensland, 4067 St. Lucia, Brisbane, Australia;(2) Department of Chemical Engineering, University of Queensland, 4067 St. Lucia, Brisbane, Australia |
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Abstract: | Summary Zymomonas mobilis was grown in batch concentrations between 200 and 400 g/l sucrose. The fermentation pattern revealed that the efficiency of sucrose hydrolysis dropped only from 94 to 78.6% whereas the efficiency with which the hydrolyzed products were converted to ethanol decreased from 94 to 43%. The ethanol yields were relatively constant for final concentrations which lay between 80 and 132 g/l. Fermentation times increased to 72 hours at the higher sucrose concentrations. Sorbitol and fructose were identified as the major by-products. Preliminary evidence suggests that the ratio between the two by-products depends on the pH of the culture medium. Results suggest the possibility of processes producing ethanol plus fructose, ethanol plus fructose and sorbitol, or ethanol plus sorbitol in a single-stage batch fermentation. |
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