Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli |
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Authors: | Gao Guanjun Tian Bing Liu Lili Sheng Duohong Shen Binghui Hua Yuejin |
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Affiliation: | Institute of Nuclear-Agricultural Science, Zhejiang University, 310029, China. |
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Abstract: | PprI, a newly identified gene switch responsible for extreme radioresistance of Deinococcus radiodurans, plays a central regulatory role in multiple DNA damage repair and protection pathways in response to radiation stress [Biochem. Biophy. Res. Commun. 306 (2003) 354]. To evaluate whether PprI also functions in the radioresistance in other organisms, D. radiodurans PprI protein (Deira-PprI) was expressed in Escherichia coli. The complemented E. coli strain showed an increase of approximately 1.6-fold radioresistance with a high dose of gamma irradiation. Immunoblotting assays showed that the expression of Deira-PprI in E. coli resulted in a significant increase in RecA protein expression following high dose ionizing radiation. The expression of Deira-PprI protein also significantly enhanced the scavenging ability of free radicals by inducing the enzymatic activity of KatG. These results indicate that exogenous expression of Deira-PprI promotes DNA repair and protection pathways and enhances the radioresistance of E. coli. |
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Keywords: | Deinoccocus radiodurans Radiation DNA damage and repair |
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