Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana |
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| Authors: | Elly Speulman David Bouchez Eric B Holub Jim L Beynon |
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| Institution: | ;1Department of Biological Sciences, Wye College, Wye, Ashford, Kent TN25 5AH, UK,,;2Laboratoire de Biologie Cellulaire, INRA, 78026 Versailles Cedex, France, and,;3Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UK |
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| Abstract: | The disease resistance genes RPS2 of Arabidopsis and N of tobacco, among other recently cloned resistance genes, share several conserved sequences. Degenerate oligonucleotide primers, based on conserved sequences in the nucleotide binding site (NBS) and a weak hydrophobic domain of RPS2 and N, were used to amplify homologous sequences from Arabidopsis thaliana. Amplification products were obtained that were similar in sequence to the disease resistance genes RPS2, RPM1, N and L6. The Arabidopsis CIC-YAC library was used to identify the position of the disease resistance homologs on the Arabidopsis genome. Their map positions could be correlated with the disease resistance loci RPS5, RAC1, RPP9, CAR1, RPP7, RPW2, RPP1, RPP10, RPP14, RPP5, RPP4, RPS2, RPW6, HRT, RPS4, RPP8, RPP21, RPP22, RPP23, RPP24 and TTR1. This method was therefore not only successful in the identification of sequences located within gene clusters that are involved in disease resistance, but could also contribute to the cloning of disease resistance genes from Arabidopsis. |
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