| 化学发光杂交进行菌落克隆的原位筛选 |
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| 引用本文： | 陈德喜,王申五,张宝春. 化学发光杂交进行菌落克隆的原位筛选[J]. 中国生物化学与分子生物学报, 1997, 13(5): 537-539 |
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| 作者姓名： | 陈德喜 王申五 张宝春 |
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| 作者单位： | 北京医科大学人民医院中心实验室 |
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| 摘 要： | 用化学发光杂交试剂代替３２Ｐ进行菌落原位筛选重组Ｐ５３阳性克隆，可减少同位素的放射性损害和污染及半衰期短等缺点．本方法实验周期短、特异性可和同位素相当，可用于重组目的基因的原位杂交筛选．
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| 关 键 词： | HRP－DNA探针 原位杂交 PCR 增强化学发光 |
| 收稿时间： | 1997-10-20 |
In situ Screening of Bacterial Colonies by ECL Method |
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| Chen De Xi Wang Shen Wu Zhang Bao Chun. In situ Screening of Bacterial Colonies by ECL Method[J]. Chinese Journal of Biochemistry and Molecular Biology, 1997, 13(5): 537-539 |
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| Authors: | Chen De Xi Wang Shen Wu Zhang Bao Chun |
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| Affiliation: | (Central laboratory,People's Hospital,Beijing Medical University,Beijing 100044 |
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| Abstract: | Enhanced chemiluminescent(ECL) reagent instead of 32 P isotope was used in the screening of bacterial colonies which contain recombinant P53 cDNA.It overcomes the disadvantages of radioactive injuries,pollution and shorter half time of 32 P isotope.Compared with radioautography,enhanced chemiluminescent autophotography needs less time and has the same specificity with the former.Therefore,it can be used conveniently in the screening of interested bacterial colonies. |
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| Keywords: | HRP DNA probe In situ hybridization PCR Enhanced chemiluminescence |
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