Evaluation of the strains of Acinetobacter and Enterobacter as potential biocontrol agents against Ralstonia wilt of tomato |
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Authors: | Qing-Yun Xue Yu Chen Shi-Mo Li Li-Feng Chen Guo-Chun Ding Da-Wei Guo Jian-Hua Guo |
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Institution: | 1. College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, Jiangsu 210095, China;2. College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;3. Huaiyin Tearcher’s college, Jiangsu Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Huaian, Jiangsu 223001, China;4. Longyan Academy of Agricultural Sciences, Longyan, Fujian 364000, China |
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Abstract: | Bacterial wilt (Ralstonia solanacearum) of tomato, Lycopersicon esculentum, causes a considerable amount of damage to tomato in Southern China. Biological control is one of the more promising approaches to reduce the disease incidence and yield losses caused by this disease. Based on antagonistic activity against R. solanacearum and three soil-borne fungal pathogens as well as biocontrol efficacy in the greenhouse, two bacterial strains Xa6 (Acinetobacter sp.) and Xy3 (Enterobacter sp.) were selected out of fourteen candidates as potential biocontrol agents. In order to find a suitable antagonist inoculation method, we compared the methods of root-dipping with soil-drenching in the aspects including rhizocompetence, biocontrol efficacy, and effect of promoting plant growth under greenhouse conditions. The drenching treatment resulted in a higher biocontrol efficacy and plant-yield increase, and this method was also easier to operate in the field on a large scale. Field trials were conducted for further evaluation of these two antagonistic strains. In both greenhouse and field experiments, the strain Xy3 had a better control effect against bacterial wilt than Xa6 did, while Xa6 caused higher biomass or yield increases. As recorded on the 75th day after treatment in two field experiments, biocontrol efficacy of Xy3 was about 65% in both field trials, and the yield increases caused by Xa6 were 32.4 and 40.7%, respectively, in the two trials. This is the first report of an Acinetobacter sp. strain used as a BCA against Ralstonia wilt of tomato. |
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Keywords: | Antagonist Ralstonia solanacearum Biological control Acinetobacter sp Root-dipping Drenching Enterobacter sp |
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