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Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detection |
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| Authors: | Peters Brock A Kan Zhengyan Sebisanovic Dragan Pujara Kanan Wang Zhiyong Hong Peter Chow Bernard Stinson Jeremy Carlton Victoria E H Pham Thinh Q Stern Howard Waring Paul Hillan Kenneth J Eberhard David A de Sauvage Frederic Zheng Jianbiao Faham Malek Seshagiri Somasekar |
| Affiliation: | Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA. |
| Abstract: | The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations in human tumors. |
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