A fucosyltransferase in teratocarcinoma stem cells. Decreased activity accompanying differentiation to parietal endoderm cells |
| |
Authors: | H Muramatsu T Muramatsu |
| |
Institution: | Department of Biochemistry, Kagoshima University School of Medicine, 1208-1, Usukicho, Kagoshima 890, Japan |
| |
Abstract: | Teratocarcinoma stem cell F9 expressed a potent fucosyltransferase activity acting on asialofetuin. A majority of the product was susceptible to alpha-L-fucosidase I from almond emulsin, indicating that the linkage formed was mainly Fuc alpha 1 leads to 3GlcNAc. The specific activity of the transferase decreased when the stem cells were induced to differentiate into parietal endoderm cells by retinoic acid and dibutyryl cyclic AMP. Furthermore, PYS-2 cell, a parietal endoderm cell line virtually lacked the transferase. The change in the fucosyltransferase activity could be correlated with cell surface changes occurring during differentiation. |
| |
Keywords: | Fucosyltransferase Glycosyltransferase Teratocarcinoma stem cell Cell surface Differentiation Parietal endoderm cell Diff-F9 F9 cells differentiated by retinoic acid and dibutyryl cyclic AMP FBP |
本文献已被 ScienceDirect 等数据库收录! |
|