PCR amplification of the 3' external transcribed and intergenic spacers of the ribosomal DNA repeat unit in three species of Saccharomyces |
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Authors: | Francis I. Molina Shung-Chang Jong John L. Huffman |
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Affiliation: | Institute of Technology, Shimizu Corporation, Etchujima, Koto-ku, Tokyo, Japan; Fermentation Research Institute, Agency of Industrial Science Technology, Tsukuba, Ibaraki, Japan |
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Abstract: | Abstract Desulfotomaculum thermobenzoicum TSB converted 4 mol pyruvate to 5 mol acetate in the absence of sulfate. The cells grown on pyruvate without sulfate showed both carbon monoxide dehydrogenase (CODH) and methylmalonyl-CoA: pyruvate transcarboxylase activities. However, considering the fermentation products, the acetogenesis from pyruvate might be conducted by CODH pathway rather than methylmalonyl-CoA pathway. Contrary to this finding, Desulfobulbus propionicus MUD fermented 3 mol pyruvate to 2 mol acetate and 1 mol propionate stoichiometrically via methylmalonyl-CoA pathway. Desulfovibrio vulgaris Marburg, which has neither the CODH pathway nor the methylmalonyl-CoA pathway, converted pyruvate to acetate, H2 and CO2 as the main products. These results indicate that the fermentation pattern of pyruvate depends on the metabolic characteristics of each sulfate-reducing bacterium. |
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Keywords: | Desulfotomaculum thermobenzoicum Desulfobulbus propionicus Desulfovibrio vulgaris Carbon monoxide dehydrogenase pathway Methylmalonyl-CoA pathway Fermentation of pyruvate Sulfate-reducing bacteria |
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