舞毒蛾谷胱甘肽S-转移酶对杨树次生物质协同溴氰虫酰胺的胁迫响应

为了明确舞毒蛾Lymantria dispar谷胱甘肽S-转移酶(GST)对杨树次生物质协同溴氰虫酰胺的胁迫响应机制，选择3种杨树次生物质(黄酮、槲皮素、芦丁)以及新型邻二苯甲酰胺类杀虫剂溴氰虫酰胺作为胁迫外源化合物，以舞毒蛾2龄幼虫为研究对象，通过人工饲料添加次生物质和溴氰虫酰胺的单剂和混剂，测定对舞毒蛾存活率、谷胱甘肽S-转移酶活性及其基因表达影响。结果表明，处理48 h后，3种联合处理组舞毒蛾幼虫的存活率显著低于对照组和各杨树次生物质单剂处理组，存活率依次为53.33%、60.00%和53.33%,各联合处理组幼虫存活率与溴氰虫酰胺处理组差异不显著。除处理6 h外，不同杨树次生物质单剂处理后GST活性均诱导增加。溴氰虫酰胺处理组在48 h内GST活性显著高于单剂处理组和对照组。除联合处理1在6 h、12 h的GST诱导活性低于溴氰虫酰胺处理组外，各联合处理组的GST诱导活性均高于溴氰虫酰胺处理组。舞毒蛾2龄幼虫取食含有不同处理的人工饲料后，其体内LdGSTe2、LdGSTs1、LdGSTs2和LdGSTz1均有所表达，且不同处理的诱导程度呈现差异。以上研究结果为杨树次生物质协同溴...

Responses of glutathione S-transferase in Lymantria dispar to poplar secondary metabolites synergistic with cyantraniliprole

In order to clarify the response mechanism of poplar secondary metabolites on glutathione S-transferase in Lymantria dispar, three poplar secondary metabolites (flavone, quercetin, and rutin) and the new o-dibenzamide insecticide (cyantraniliprole) were chosen as stress xenobiotics. The 2nd instar larvae of L. dispar were used to investigate the effects of single and joint of poplar secondary metabolites and cyantraniliprole on survival rates, enzyme activity and gene expression. After treatment for 48 h, the results showed that the survival rate of larvae in the three combined treatment groups was significantly lower than that of the control group and each single poplar secondary substance treatment group with the survival rates of 53.33%, 60.00% and 53.33%, respectively. For survival rate, there was no significant difference between three combined treatment groups and the cyantraniliprole-treated group. Except for 6 h of treatment, GST activity was induced by different single poplar secondary metabolite. The GST activity in cyantraniliprole-treated group was significantly higher than the control group and single secondary metabolite group during 48 h. Except the GST activities in the mixture treatment I were lower than cyantraniliprole treatment at 6 h and 12 h, the GST activity in mixture I and mixture II treatments was higher than that of the cyantraniliprole treatment. The LdGSTe2, LdGSTs1, LdGSTs2 and LdGSTz1 genes differentially expressed in 2nd instar larvae of L. dispar under different treatments. These results will provide theoretical foundation for the controlling L. dispar by combined usage of poplar secondary substances and cyantraniliprole, as well as provide a reference basis for the rational usage of insecticides in practice.