Changes in jasmonates and 12-oxophytodienoic acid contents of <Emphasis Type="Italic">Medicago sativa</Emphasis> L. during somatic embryogenesis |
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Authors: | Email author" target="_blank">Izabela?RuduEmail author Ewa?K?pczy ska Jan?K?pczy ski Claus?Wasternack Otto?Miersch |
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Institution: | (1) Department of Plant Physiology and Biotechnology, Szczecin University, W ska 13, 71-415 Szczecin, Poland;(2) Department of Natural Product Biotechnology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle Saale, Germany |
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Abstract: | Jasmonic acid (JA), its methyl ester (MeJA) and the biosynthetic precursor 12-oxophytodienoic acid (OPDA) were detected quantitatively
during somatic embryogenesis of Medicago sativa L. Using GC-MS analysis, these compounds were found in initial explants, in calli and in somatic embryos in the nanogram
range per gram of fresh weight. In distinct stages of somatic embryogenesis, JA and 12-OPDA accumulated preferentially in
cotyledonary embryos. Initial explants exhibited about five-fold higher JA content than OPDA content, whereas in other stages
OPDA accumulated predominantly. These data suggest that also in embryogenic tissues OPDA and JA may have individual signalling
properties. |
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Keywords: | jasmonates Medicago sativa somatic embryogenesis |
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