Evidence for clustered mannose as a new ligand for hyaluronan- binding protein (HABP1) from human fibroblasts |
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Authors: | Kumar R Choudhury N R Salunke D M Datta K |
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Institution: | (1) Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, 110 067 New Delhi, India;(2) Genetic Engineering Unit, Jawaharlal Nehru University, 110 067 New Delhi, India;(3) National Institute of Immunology, Aruna AsafAli Marg, 110 067 New Delhi, India |
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Abstract: | We have earlier reported that overexpression of the gene encoding human hyaluronan-binding protein (HABP1) is functionally
active, as it binds specifically with hyaluronan (HA). In this communication, we confirm the collapse of the filamentous and
branched structure of HA by interaction with increasing concentrations of recombinant-HABP1 (rHABP1). HA is the reported ligand
of rHABP1. Here, we show the affinity of rHABP1 towards D-mannosylated albumin (DMA) by overlay assay and purification using
a DMA affinity column. Our data suggests that DMA is another ligand for HABP1. Furthermore, we have observed that DMA inhibits
the binding of HA in a concentration-dependent manner, suggesting its multiligand affinity amongst carbohydrates. rHABP1 shows
differential affinity towards HA and DMA which depends on pH and ionic strength. These data suggest that affinity of rHABP1
towards different ligands is regulated by the microenvironment. |
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Keywords: | Hyaluronan hyaluronan binding protein D-mannosylated albumin |
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