Transport of leucine in the cyanobacterium Anabaena variabilis |
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Authors: | Teresa Thiel |
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Affiliation: | (1) Department of Biology, University of Missouri-St. Louis, 63121 St. Louis, MO, USA |
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Abstract: | The amino acid leucine was transported by the cyanobacterium Anabaena variabilis. The Km for transport was 10.8 M; the Vmax was 8.7 nmoles min–1 mg–1 chlorophyll a. Transport of leucine was energy dependent: uptake of leucine was inhibited in the dark, and by DCMU and cyanide. Transport was neither dependent on nor enhanced by Na+. Prior growth of cells with leucine did not repress transport of [14C]-leucine. Alanine, glycine, valine, and methionine were strong competitive inhibitors of leucine uptake; serine, threonine, isoleucine, norleucine, and d-alanine competitively inhibited to a lesser degree. Other amino acids or amino acid analogues, including d-leucine, -aminoisobutyrate, and d-serine did not inhibit the transport of leucine.Abbreviations Chl a chlorophyll a - DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea - TES N-tris(hydroxymethyl)-2-aminoethane-sulfonic acid - TCA trichloroacetic acid - Tris N-tris(hydroxymethyl)aminoethane |
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Keywords: | Amino acid transport Leucine Threonine Cyanobacteria Blue-green algae |
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