Ultrastructural organization of nucleoproteins during aging of cultured human embryonic fibroblasts |
| |
Authors: | F. Puvion-Dutilleul A. Macieira-Coelho |
| |
Affiliation: | 1. Institut de Recherches Scientifiques sur le Cancer, CNRS France;2. Institut de Cancérologie et d''Immunogénétique, INSERM, U-50, F-94802-Villejuif Cedex, France |
| |
Abstract: | Analysis of nucleoproteins in resting human embryonic fibroblasts in vitro at different population doubling levels (PDL) using electron microscopy revealed the disappearance of non-nucleolar ribonucleoprotein structures at high PDL, the nucleoli became larger and the filamentous masses containing the nascent nucleolar RNA displayed a fibrillo-granular pattern which has never been described previously. In addition, conventional fixation revealed the disappearance of most of the stainable chromatin whose threads were unusually spaced and shortened specially at the nuclear surface after loosening. We interpret these changes in chromatin organization as the consequence of the alkali-sensitive sites that accumulate during senescence. |
| |
Keywords: | To whom offprint requests should be sent. |
本文献已被 ScienceDirect PubMed 等数据库收录! |