Discrimination of Repetitive Sequences Polymorphism in Secale cereale by Genomic In Situ Hybridization-Banding |
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Authors: | Jian-Ping Zhou Zu-Jun Yang Guang-Rong Li Cheng Liu Zheng-Long Ren |
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Affiliation: | ( Shcool of Life Science and Technology, University of Electronic Science and Technology of China;, Chengdu 610054, China;State Key Laboratory for Plant Genetics and breeding, Sichuan Agricultural University;, Ya'an 625014, China) |
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Abstract: | Genomic in situ hybridization banding (GISH-banding), a technique slightly modified from conventional GISH, was used to probe the Chinese native rye (Secale cereale L.) DNA, and enabled us to visualize the Individual rye chromosomes and create a universal reference karyotype of the S. cereale chromosome 1R to 7R. The GISH-banding approach used in the present study was able to discriminate S. cereale chromosomes or segments in the wheat (Triticum aeativum L.) background, including the Triticale, wheat-rye addition and translocation lines. Moreover, the GISH-banding pattern of S.cereale subsp. Afghanicum chromosomes was consistent with that of Chinese native rye cv. Jingzhou rye; whereas the GISH-banding pattem of Secale vavilovli was different from that of S. cereale, indicating that GISH-banding can be used to study evolutionary polymorphism in species or subspecies of Secale. In addition, the production and application of GISH-banding to the study of adenine-thymine-riched heterochromatin is discussed. |
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Keywords: | adenine-thymine-riched heterochromatin genomic in situ hybridization-banding karyotype repetitive sequences Secale cereale. |
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