Androgen receptor in rat Harderian and submandibular glands |
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| Authors: | Ya-Hua Zhuang Merja Bl?uer Heimo Syv?l? Merja Laine and Pentti Tuohimaa |
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| Institution: | (1) Molecular Endocrinology Research Unit and Graduate School of Steroid Research, Department of Anatomy, Tampere University Medical School, FIN-33101 Tampere, Finland;(2) Institute of Medical Technology, University of Tampere, Finland;(3) Institute of Dentistry, University of Turku, Finland |
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| Abstract: | Summary Androgens regulate the development and sexual dimorphism of rodent Harderian and submandibular glands. This effect is believed
to be mediated by the androgen receptor. Immunohistochemistry and immunoblotting were carried out to study the receptor in
normal, castrated and dihydrotestosterone-supplemented rat Harderian and submandibular glands. Immunohistochemically, the
most intense nuclear staining was observed in the acinar cells of the submandibular glands, followed by intercalated duct
cells. The granular convoluted tubules showed weak immunostaining and the striated ducts were negative. In the Harderian gland,
nuclear staining was seen in both type I and II secretory cells. Castration and treatment had no effect on the expression
of the androgen receptor protein in either gland. A 110 K androgen receptor signal was detected by immunoblotting in the Harderian
gland but not in the submandibular gland. An experiment was designed to explore the possible effect of proteinases on the
receptor protein in the homogenate of submandibular gland. Our results demonstrate the cell-specific location of the receptor
in Harderian and submandibular glands, and show that the expression of the receptor protein is androgen-independent. |
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