Glucosamine-mediated detoxification of p-benzoquinone and its removal by chitosan |
| |
Authors: | Sang Ho Park Oh Sung Kwon Chang Won Choi Chang-Jin Kim |
| |
Affiliation: | (1) Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon, 305-600, Korea;(2) Department of Biology, Pai Chai University, Taejon, 302-735, Korea |
| |
Abstract: | p-Benzoquinone non-enzymatically reacted with d-glucosamine at physiological pH and moderate temperature. The reaction of p-benzoquinone with glucosamine was signaled by changes in the UV and visible spectra. The reactivity proceeded fastest at pH values above 7, with a sharp drop from pH 6.5 to 7.0, and the reaction was negligible in acidic conditions. The order of reactivity of amino sugars was d-mannosamine > d-glucosamine > d-galactosamine. From the reaction mixture, four conversion products were isolated and none was toxic to Escherichia coli even at 500–700 g ml–1, while p-benzoquinone was cytotoxic to E. coli at 20 g ml–1. Chitosan could react with p-benzoquinone efficiently and remove this toxicant in aqueous solution. |
| |
Keywords: | chitosan detoxification p-benzoquinone removal |
本文献已被 SpringerLink 等数据库收录! |
|