Detection and preliminary characterization of extracellular proteolytic activities of the haloalkaliphilic archaeon Natronococcus occultus |
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Authors: | C A Studdert Rosana E De Castro Karina Herrera Seitz Jorge J Sánchez |
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Institution: | (1) Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, C.C. 1245, Mar del Plata 7600, Argentina Tel. +54-23-753030; Fax +54-23-753150 e-mail: studdert@mdp.edu.ar, AR |
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Abstract: | Extracellular proteolytic activity was detected in the haloalkaliphilic archaeon Natronococcus occultus as the culture reached the stationary growth phase. Proteolytic activity was precipitated with ethanol and subjected to a
preliminary characterization. Optimal conditions for activity were attained at 60° C and 1–2 M NaCl or KCl. Gelatin zymography
in the presence of 4 M betaine revealed a complex pattern of active species with apparent molecular masses ranging from 50
to 120 kDa. Experiments performed with inhibitors of the various groups of proteases indicated that the extracellular proteolytic
enzymes of N. occultus are of the serine type. Individual protein species showed some differences in salt and thermal stability.
Received: 10 January 1997 / Accepted: 27 June 1997 |
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Keywords: | Archaea Haloalkaliphiles Natronococcus occultus Proteolytic activity Gelatin zymography Betaine |
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