SO2衍生物诱发蚕豆根尖细胞微核和后期异常的研究

研究SO₂体内衍生物--亚硫酸钠和亚硫酸氢钠混合液(3:1 mmol·L^-1/mmol·L^-1)诱发蚕豆根尖细胞微核和后期异常的效应。结果表明:SO₂衍生物处理可诱发蚕豆根尖间期细胞微核和核芽,使分裂后期出现多种染色体异常,如断片、桥以及滞后染色体等。异常细胞中以微核细胞和染色体断裂细胞居多。在一定浓度范围内,细胞异常率与处理液浓度之间表现正的线性相关。这些研究结果表明,蚕豆根尖间期微核和后期染色体异常有可能用作检测SO₂污染的生物剂量计。

MICRONUCLEUS AND ANAPHASE ABERRATIONS IN VICIA FABA ROOT TIPS INDUCED BY THE DERIVATIVES OF SULFUR DIOXIDE

The micronucleus and anaphase aberrations in Vicia faba root tips induced by a mixture of sodium sulfite and sodium bisulfite 3:1, a solution form of sulfur dioxide, were investigated. The results showed that sodium bisulfite solution caused micronucleus and nuclear buds in interphase cells and also anaphase aberrations such as chromosomal segment, bridge and lagging in division cells. The abnormal cells with micronuclei or chromosomal breakage were the most of all aberrant cells in the Vicia root tips after exposure to bisulfite. The frequencies of cells with various aberrations had positive response to the treated concentrations. These results indicate that SO 2 can induce cytogenetic damage in plant cells and both frequencies of Vicia micronucleus and anaphase aberrations could be used as the biological dosimeters to monitor SO 2 pollutant in the environment.