Nitrobenzylthioinosine-insensitive uridine transport in human lymphoblastoid and murine leukemia cells |
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Authors: | J A Belt |
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Affiliation: | Division of Biochemical and Clinical Pharmacology St. Jude Children''s Research Hospital Memphis, Tennessee 38101 USA |
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Abstract: | Both human lymphoblastoid (RPMI 6410) and murine leukemia (L1210) cells were found to have a component of uridine transport which is insensitive to the nucleoside transport inhibitor nitrobenzylthioinosine (NBMPR). In both cell lines NBMPR-insensitive uridine transport is inhibited by other nucleosides and by the sulfhydryl reagent p-chloromercuribenzenesulfonate. In RPMI 6410 cells NBMPR-insensitive transport accounts for only 2% of the initial rate of uridine transport. In contrast, 20% of the initial rate of transport of L1210 cells is insensitive to NBMPR, and uridine uptake over longer periods (10 min) is completely insensitive to NBMPR. |
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