| 毕赤酵母发酵生产T4溶菌酶的中试研究 |
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| 引用本文： | 吴家鑫,余志强,王华丽,葛辛玫,齐鹏,郑应华,刘德虎,宋敏,张国栋,曹芹.毕赤酵母发酵生产T4溶菌酶的中试研究[J].中国生物工程杂志,2010,30(12):49-52. |
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| 作者姓名： | 吴家鑫 余志强 王华丽 葛辛玫 齐鹏 郑应华 刘德虎 宋敏 张国栋 曹芹 |
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| 作者单位： | 1. 中牧实业股份有限公司兽药研究所 北京 100091;
2. 中国农业科学院生物技术研究所 北京 100081 |
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| 基金项目： | "十一五"国家科技支撑计划 |
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| 摘 要： | 研究了T4溶菌酶的中试发酵放大生产。在200L发酵罐中毕赤酵母诱导表达T4溶菌酶蛋白,发酵液蛋白表达量达到1.69g/L,酶活达到192U/mg;制得冻干酶粉211.1g。经测定粗酶粉中T4溶菌酶的含量达到68.9%,酶活为182U/mg,总收率达到66%,成功建立了一条T4溶菌酶的中试发酵生产工艺线路。
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| 关 键 词： | T4溶菌酶 中试 毕赤酵母 |
| 收稿时间： | 2010-08-18 |
| 修稿时间： | 2010-09-26 |
The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris |
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| WU Jia-xin,YU Zhi-qiang,WANG Hua-li,GE Xin-mei,QI Peng,ZHENG Ying-hua,LIU De-hu,SONG Min,ZHANG Guo-dong,CAO Qin.The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris[J].China Biotechnology,2010,30(12):49-52. |
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| Authors: | WU Jia-xin YU Zhi-qiang WANG Hua-li GE Xin-mei QI Peng ZHENG Ying-hua LIU De-hu SONG Min ZHANG Guo-dong CAO Qin |
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| Institution: | 1. Institute of Veterinary Drug, China Animal Husbandry Industry Co.,Ltd,Beijing 100091, China;
2. Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | T4 lysozyme pilot-scale fermentation was studied. T4 lysozyme was induced and expressed by Pichia pastoris in the 200L fermentor. The yield of target protein was 1.69g /L with 192U/mg enzyme activity in the broth. The enzyme protein was freeze-dried. The weight of enzyme powder was 211.1g. T4 lysozyme content was 68.9%, and activity was 182U/mg in enzyme powder. The total yield was 66%. The T4 lysozyme pilot-scale process line was established successfully. |
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| Keywords: | T4 lysozyme Pilot-scale Pichia pastoris |
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