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毕赤酵母发酵生产T4溶菌酶的中试研究
引用本文:吴家鑫,余志强,王华丽,葛辛玫,齐鹏,郑应华,刘德虎,宋敏,张国栋,曹芹. 毕赤酵母发酵生产T4溶菌酶的中试研究[J]. 中国生物工程杂志, 2010, 30(12): 49-52
作者姓名:吴家鑫  余志强  王华丽  葛辛玫  齐鹏  郑应华  刘德虎  宋敏  张国栋  曹芹
作者单位:1. 中牧实业股份有限公司兽药研究所 北京 100091;2. 中国农业科学院生物技术研究所 北京 100081
基金项目:"十一五"国家科技支撑计划
摘    要:研究了T4溶菌酶的中试发酵放大生产。在200L发酵罐中毕赤酵母诱导表达T4溶菌酶蛋白,发酵液蛋白表达量达到1.69g/L,酶活达到192U/mg;制得冻干酶粉211.1g。经测定粗酶粉中T4溶菌酶的含量达到68.9%,酶活为182U/mg,总收率达到66%,成功建立了一条T4溶菌酶的中试发酵生产工艺线路。

关 键 词:T4溶菌酶  中试  毕赤酵母  
收稿时间:2010-08-18
修稿时间:2010-09-26

The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris
WU Jia-xin,YU Zhi-qiang,WANG Hua-li,GE Xin-mei,QI Peng,ZHENG Ying-hua,LIU De-hu,SONG Min,ZHANG Guo-dong,CAO Qin. The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris[J]. China Biotechnology, 2010, 30(12): 49-52
Authors:WU Jia-xin  YU Zhi-qiang  WANG Hua-li  GE Xin-mei  QI Peng  ZHENG Ying-hua  LIU De-hu  SONG Min  ZHANG Guo-dong  CAO Qin
Affiliation:1. Institute of Veterinary Drug, China Animal Husbandry Industry Co.,Ltd,Beijing 100091, China;2. Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract:T4 lysozyme pilot-scale fermentation was studied. T4 lysozyme was induced and expressed by Pichia pastoris in the 200L fermentor. The yield of target protein was 1.69g /L with 192U/mg enzyme activity in the broth. The enzyme protein was freeze-dried. The weight of enzyme powder was 211.1g. T4 lysozyme content was 68.9%, and activity was 182U/mg in enzyme powder. The total yield was 66%. The T4 lysozyme pilot-scale process line was established successfully.
Keywords:T4 lysozyme  Pilot-scale  Pichia pastoris
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