| CSPGs在大鼠脊髓损伤后的表达及其与GFAP的关系 |
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| 引用本文： | 安俊,段丽,张欲凯,张海峰,刘新秦,沈学锋.CSPGs在大鼠脊髓损伤后的表达及其与GFAP的关系[J].现代生物医学进展,2010,10(24). |
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| 作者姓名： | 安俊 段丽 张欲凯 张海峰 刘新秦 沈学锋 |
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| 作者单位： | 1. 第四军医大学预防医学院军队劳动与环境卫生学教研室
2. 第四军医大学神经科学研究所,陕西西安,710032 |
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| 基金项目： | 陕西省社发攻关项目(2006K152G1) |
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| 摘 要： | 目的:观察脊髓损伤后CSPGs的表达及其与GFAP的关系。方法:成年雄性SD大鼠25只,随机分为对照组和损伤组,损伤组分脊髓挤压损伤后0h、72h、1w、4w组,运用免疫荧光双重染色方法观察CSPGs与GFAP的表达。结果:挤压伤后损伤部位的CSPGs和GFAP的表达均增高,但二者的变化趋势并不一样。其中CSPGs从损伤后表达开始增高,此后一直增加,并在1w至4w时逐渐稳定,主要分布逐渐集中于损伤部位;星形胶质细胞的免疫反应也逐渐增加,其分布逐渐集中于损伤区域的边缘,逐渐形成胶质瘢痕界膜。损伤1w至4w,损伤区域内几乎没有了星形胶质细胞表达,但仍留有大量的CSPGs。结论:早期抑制星形胶质细胞分泌CSPGs,可以防止在损伤部位沉积大量的CSPGs,从而减小其对再生纤维的抑制作用。
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| 关 键 词： | 脊髓损伤 硫酸软骨素蛋白多糖 星形胶质细胞 胶质瘢痕 |
The expression of chondroitin sulphate proteoglycans and its relationship with astrocyte after rat spinal cord compression injury |
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| AN Jun,DUAN Li,ZHANG Yu-kai,ZHANG Hai-feng,LIU Xin-qin,SHEN Xue-feng.The expression of chondroitin sulphate proteoglycans and its relationship with astrocyte after rat spinal cord compression injury[J].Progress in Modern Biomedicine,2010,10(24). |
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| Authors: | AN Jun DUAN Li ZHANG Yu-kai ZHANG Hai-feng LIU Xin-qin SHEN Xue-feng |
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| Institution: | AN Jun1,DUAN Li2,ZHANG Yu-kai2,ZHANG Hai-feng2,LIU Xin-qin1,SHEN Xue-feng1(1 Department of Occupational and Environmental Health,Faculty of Military Preventive Medicine,2 Institute of Neuroscience,The Fourth Military Medical University,Shanxi,Xi'an 710032,China) |
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| Abstract: | Objective: To investigate the time course and distribution of chondroitin sulphate proteoglycans(CSPGs) and glial fib-rillary acidic protein(GFAP).Methods:A total of 25 Sprague-Dawley rats were divided into control and injured group.At 0h,72h,1w,4w after compression injuries of the spinal cord,the expression of CSPGs and GFAP were detected by double-stained immunofluores-cence analysis.Results: The expression of both CSPGs and GFAP within the injury area of the spinal cord were increased,but the time course... |
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| Keywords: | spinal cord injury chondroitin sulphate proteoglycans(CSPGs) astrocyte glial scar |
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