| 光动力疗法对体外培养人胰腺癌细胞株PANC-1作用 |
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| 引用本文： | 徐晓军,张振玉. 光动力疗法对体外培养人胰腺癌细胞株PANC-1作用[J]. 现代生物医学进展, 2010, 10(24) |
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| 作者姓名： | 徐晓军 张振玉 |
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| 作者单位： | 南京医科大学附属南京第一医院消化科,江苏,南京,210006 |
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| 基金项目： | 南京市科技局基金发展项目(2006ZD0117) |
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| 摘 要： | 目的:探讨血卟啉注射液(Hematoporphyrin Derivative,HPD)光动力疗法(Photodynamic Therapy,PDT)对体外培养的人胰腺癌细胞株PANC-1的生物作用。方法:实验分为4组,空白对照组、单纯HPD组、单纯光照组及HPD+PDT组。采用MTT法检测光动力作用后细胞的存活率,并用Annexin V-FITC/P I双染法检测其凋亡率。结果:在光敏剂浓度为5mg/L,光照剂量为10J/cm2时,光动力对PANC-1细胞达到最佳的实验效果,与对照组相比差异有显著性。在此实验参数条件下,流式细胞术(FCM)检测各组人胰腺癌细胞PANC-1凋亡率:HPD+PDT实验组达(36.40±4.21)%,明显高于单纯HPD(6.76±0.44)%,单纯PDT组(8.30±0.32)%及空白组(5.00±0.53)%三个对照组(P<0.05),三个对照组间差异无统计学意义(P>0.05)。结论:PDT光动力作用对体外培养人胰腺癌细胞PANC-1有明确抑制效应,并与HPD浓度及光照强度相关。
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| 关 键 词： | 光动力疗法 胰腺癌细胞 凋亡 |
Effect of photodynamic therapy on human pancreatic cancer cell line PANC-1 cultured in vitro |
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| XU Xiao-jun,ZHANG Zhen-yu. Effect of photodynamic therapy on human pancreatic cancer cell line PANC-1 cultured in vitro[J]. Progress in Modern Biomedicine, 2010, 10(24) |
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| Authors: | XU Xiao-jun ZHANG Zhen-yu |
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| Affiliation: | XU Xiao-jun,ZHANG Zhen-yu(Department of Gastroenterology,Nanjing First Hospital Affiliated to Nanjing medical university,Nanjing,210006,China) |
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| Abstract: | Objective: To investigate the effect of HPD injection(Hematoporphyrin Derivative,HPD) Photodynamic Therapy(Photodynamic Therapy,PDT) on cultured human pancreatic cancer cell line PANC-1.Methods: There were 4 groups: control group,simply HPD group,the simple light group and HPD + PDT group.The cell survival was detected by MTT assay after photodynamic,and the apoptosis was detected by double staining with Annexin V-FITC/PI.Results: It achieved the best experimental results of the PANC-1 cells in the photosen... |
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| Keywords: | Photodynamic therapy Pancreatic cancer cells Apoptosis |
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