Diethylamine extraction of proteins and peptides isolated with a mono-phasic solution of phenol and guanidine isothiocyanate |
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| Authors: | Nolan Rebecca L Teller Jan K |
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| Affiliation: | Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, USA. |
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| Abstract: | Representative extraction of both RNA and protein from a single biological sample is required for reliable assessment of coordinated changes in gene and protein expression. Such a simultaneous extraction can be performed by using Trizol Reagent. Here, we demonstrate that, as an alternative to SDS, 2% diethylamine is an effective solvent, which can be conveniently used in extraction of Trizol-isolated proteins from various tissues. Diethylamine provides efficient extraction of proteins and compatibility with a variety of common downstream analytical applications. |
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| Keywords: | Aβ, amyloid β peptide APP, amyloid precursor protein CHAPS, 3-[(3-cholamidopropyl)-dimethylammonio] propanesulfonate DC, deoxycholic acid DEA, diethylamine SDS, sodium dodecyl sulfate. |
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