Direct cloning of the human genomic apolipoprotein E gene using magnetic separation of single-stranded DNA |
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Authors: | E Hornes T Hultman T Moks M Uhlén |
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Institution: | Dynal A.S., Oslo. |
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Abstract: | Solid-phase methods can be used for direct cloning of in vitro-amplified genomic DNA with high efficiency, without the need for restriction enzymes or ligase. Single-stranded DNA fragments are generated by magnetic separation and are simply mixed with single-stranded vector to form gap-duplex molecules that can be readily transformed. This strategy, in combination with direct solid-phase DNA sequencing, was used to analyze individual alleles in the human apolipoprotein E locus. |
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