几种蚊虫线粒体DNA-16SrRNA序列及其相互关系的研究

测定我国尖音库蚊复合组4亚种（尖音库蚊、淡色库蚊、致倦库蚊和骚扰库蚊）、三带喙库蚊、白纹伊蚊和中华按蚊的线粒体DNA 16S rRNA基因（mtDNA-16S rRNA)序列，发现我国尖音库蚊复合组4亚种mtDNA-16S rRNA序列基本一致，长度为555bp(554bp)，GC含量为25．41％。该序列与其他3种蚊虫在种间存在差异，与三带喙库蚊的种间差异为0．54％；与白纹伊蚊的种间差异为5．77％；与中华按蚊的种间差异为9．62％。分子系统关系分析表明该序列与传统分类系统的同属或同亚科种类近似性相一致。

mtDNA-16S rRNA SEQUENCES OF THE CULEX PIPIENS COMPLEX AND THEIR PHYLOGENETIC ANALYSES

The 16S rRNA gene fragments of mitochondrial DNA were sequenced in the Cx. pipiens complex ( Cx. pipiens pipiens,Cx. pipiens quinquefasciatus,Cx. pipiens pallens,Cx. pipipens molestus), Cx. tritaeniorhynchus, Ae. albopictus and An. sinensis. No significant variability was found in 16S rRNA gene fragments among the mosquitoes of Cx. pipiens complex. The sequence length in Cx. pipiens complex was 555 (554) bp, and the GC content was 25 41%. There were some differences in the sequences of 16S rRNA gene fragments between the Cx. pipiens complex and the other 3 species of mosquitoes (0 54% with Cx. tritaeniorhynchus , 5 77% with Ae. albopic tus , 9 62% with An. sinensis ). The results of molecular phylogenetic analyses indicated that the similarity of these sequences was consonant with that of the species in the same genus (subfamily) in the traditional taxonomic system.