Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells |
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Authors: | Kuga Takahisa Nakayama Yuji Hoshino Masaki Higashiyama Yukihiro Obata Yuuki Matsuda Daisuke Kasahara Kousuke Fukumoto Yasunori Yamaguchi Naoto |
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Affiliation: | Department of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8675, Japan. |
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Abstract: | Src-family tyrosine kinases (SFKs) play an important role in mitosis. Despite overlapping expression of multiple SFK members, little is known about how individual SFK members are activated in M phase. Here, we examined mitotic activation of endogenous c-Src, c-Yes, and Lyn, which are co-expressed in HeLa cells. c-Src, c-Yes, and Lyn were activated at different levels in M phase, and the activation was inhibited by Cdc2 inactivation. Mitotic c-Src and c-Yes exhibited normal- and retarded-electrophoretic-mobility forms on SDS-polyacrylamide gels, whereas Lyn did not show mobility retardation. Like c-Src, the retardation of electrophoretic mobility of c-Yes was caused by Cdc2-mediated phosphorylation. The normal- and retarded-mobility forms of c-Src were comparably activated, but activation of the retarded-mobility form of c-Yes was higher than that of the normal-mobility form of c-Yes. Thus, these results suggest that endogenous c-Src, c-Yes, and Lyn are differentially activated through Cdc2 activation during M phase. |
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Keywords: | Src-family tyrosine kinase c-Src c-Yes Lyn Fyn Cdc2 Mitosis M phase Phosphorylation Electrophoretic mobility |
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