| 沙门氏菌中dnd基因簇的缺失与异源表达 |
| |
| 引用本文： | 徐铁刚,姚 芬,周秀芬,由德林,邓子新.沙门氏菌中dnd基因簇的缺失与异源表达[J].微生物学通报,2009,36(7):0968-0973. |
| |
| 作者姓名： | 徐铁刚 姚 芬 周秀芬 由德林 邓子新 |
| |
| 作者单位： | 上海交通大学生命科学技术学院,上海,200030 |
| |
| 基金项目： | 国家自然科学基金项目(No. 30670032, 30770458); 国家863计划项目(No. 2006AA02Z224) |
| |
| 摘 要： | 沙门氏菌Salmonella enterica serovar Cerro 87是一株从鸡场分离到的菌株, 其DNA骨架上的磷硫酰化导致了在高压脉冲电泳过程中DNA降解(DNA degradation, Dnd表型)。本研究采用SacB所介导的负筛选系统, 在该菌株中成功缺失了dnd基因簇, 构建了突变株XTG103, 该突变株不再具有Dnd表型。通过异丙基-b-D硫代半乳糖苷(IPTG)诱导启动子PlacZ的转录可以调控DNA磷硫酰化修饰的dnd基因簇的异源表达。
|
| 关 键 词： | 沙门氏菌 DNA磷硫酰化 DNA降解 基因缺失 |
Deletion of dnd Gene Cluster in Salmonella enterica and Heterogenous Expression |
| |
| XU Tie-Gang,YAO Fen,ZHOU Xiu-Fen,YOU De-Lin and DENG Zi-Xin.Deletion of dnd Gene Cluster in Salmonella enterica and Heterogenous Expression[J].Microbiology,2009,36(7):0968-0973. |
| |
| Authors: | XU Tie-Gang YAO Fen ZHOU Xiu-Fen YOU De-Lin and DENG Zi-Xin |
| |
| Institution: | School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China;School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China;School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China;School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China;School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China |
| |
| Abstract: | Salmonella enterica serovar Cerro 87, which was isolated from a commercial egg-producing farm, has a phosphorothioated DNA backbone resulting DNA degradation(Dnd) during the |
| |
| Keywords: | Salmonella DNA Phosphorothioation DNA degradation Gene deletion |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
