Bacillus stearothermophilus plasmid pSTK1 replicon is functional in Escherichia coli |
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| Authors: | Issay Narumi Noriyuki Nakayama Shinya Nakamoto Hiroshi Kihara |
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| Institution: | (1) Physics Laboratory, Department of Liberal Arts, Kansai Medical University, 18-89 Uyamahigashi, Hirakata, 573 Osaka, Japan;(2) NEC Corporation, 4-1-1 Miyazaki, Miyamae-ku, Kawasaki, 216 Kanagawa, Japan;(3) Present address: Biotechnology Laboratory, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, 1233 Watanuki, Takasaki, 370-12 Gunma, Japan |
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| Abstract: | Summary A kanamycin-resistant plasmid possessing a thermostable replicon derived from Bacillus stearothermophilus cryptic plasmid pSTK1 was constructed. The plasmid could transform not only B. stearothermophilus and Bacillus subtilis, but also Gram-negative Escherichia coli. The behavior of the plasmid in the hosts was examined. The plasmid was stably maintained even at 67°C in B. stearothermophilus without selective pressure. During the plasmid replication, single-stranded DNA (ssDNA) intermediates were found in E. coli, while these were not found in B. subtilis. |
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