Properties of the Probiotic Strain Lactobacillus plantarum 8-RA-3 Grown in a Biofilm by Solid Substrate Cultivation Method |
| |
Authors: | Nina A Ushakova Vyacheslav M Abramov Valentin S Khlebnikov Alexandr M Semenov Boris B Kuznetsov Anna A Kozlova Alexey V Nifatov Vadim K Sakulin Igor V Kosarev Raisa N Vasilenko Marina V Sukhacheva Vyacheslav Melnikov |
| |
Institution: | 1. A.N. Severtsov Institute of Ecology and Evolution, Leninsky prospect, 33, 119071, Moscow, Russia 4. Institute of Immunological Engineering, Lyubuchany, Chekhov District, 142380, Moscow Region, Russia 2. Moscow State University, Lenin??s Hills, 1/12, 119992, Moscow, Russia 3. ??Bioengineering?? Centre, Prospekt 60-Letiya Oktyabrya, 7/1, 117312, Moscow, Russia 5. International Science and Technology Center, Krasnoproletarskaya ulitsa, 32-34, 127473, Moscow, Russia
|
| |
Abstract: | The biofilm formation took place in 48?h within the solid substrate cultivation of Lactobacillus plantarum 8-RA-3 strain on the wheat bran saturated with the MRS medium. The drying of the bran fermented by lactobacilli resulted in a decrease in the number of colony-forming units (CFU) from 23.0?×?108 to 6.9?×?105?CFU/g in daily samples and to less than 104?CFU/g in 2- and 3-day samples. However, according to the fluorescence-based live/dead assay data, more than 40?% of the non-cultured bacteria were viable. As a result of mice kept on a diet with the introduction of bran fermented by Lact. plantarum 8-RA-3 for 72?h into the fodder, a recovery of normal level of intestinal lactobacilli, inhibited by administration of antibiotic was noted. The strain genetically identical to the Lact. plantarum 8-RA-3 was isolated from the feces of these mice. The results indicate that solid substrate cultivated Lact. plantarum 8-RA-3 strain formed a biofilm. Once dried and transferred into a non-cultured state, biofilm cells retained its viability and biological activity. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|