基于航空煤油细菌检测的触变显色培养基的制备

利用正交试验测定了三种凝胶在不同配比下的触变性,并添加营养物质葡萄糖、蛋白胨、NaCl和显色剂TTC(四氮唑红),得到具有触变性的显色培养基,可直接用于航空煤油样品的检测。结果表明:黄原胶0.9%、琼脂粉0.4%和卡拉胶0.2%配伍使用,触变效果最好,其中影响因素顺序依次为黄原胶、卡拉胶、琼脂;培养基中TTC的最佳浓度范围为0.0008%～0.002%,能使细菌染成红色,并对菌的生长影响很小。

Preparation of thixotropic color medium based on bacteria in aviation kerosene

The thixotropy of three gels with different proportions were determined by means of orrhogonal test, and glucose, peptone, NaCl and TTC （red tetrazolium） were added to get the chromogenic medium with thixotropy. It could be directly used for detecting aviation kerosene sample. The results showed that the effects of compatibility with xanthan gum （0.9%）, agar （0.4%） and carrageenan （0.2%） were the best. The sequence of factors was xanthan gum, carrageenan and agar. The optimum concentration range of TTC in culture medium was from 0. 0008% to 0. 002%, in which the bacteria could be dyed red and be affected little in growth.