Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation |
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Authors: | Toshihiko Saheki Hitomi Ito Akihiro Sekiguchi Atsuyoshi Nishina Toshihiro Sugiyama Takashi Izumi Itaru Kojima |
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Institution: | (1) Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Kiryu Gunma, 376-8515, Japan;(2) Gunma Industrial Technology Center, Maebashi, Japan;(3) Department of Biochemistry, Akita University School of Medicine, Akita, Japan;(4) Department of Molecular Biochemistry, Gunma University Graduate School of Medicine, Maebashi, Japan;(5) Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan |
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Abstract: | To understand the molecular mechanism underlying vigorous proliferative activity of hepatic stem-like (HSL) cells, we performed
two-dimensional electrophoresis to identify the proteins statistically more abundant in rapidly growing undifferentiated HSL
cells than in sodium butyrate-treated differentiated HSL cells. Matrix-assisted laser desorption/ionization-time of flight
mass spectrometry and Mascot search identified 6 proteins including prohibitin, vimentin, ezrin, annexin A3, acidic ribosomal
phosphoprotein P0 and Grp75. Prohibitin and vimentin control the mitogen-activated protein (MAP) kinase pathway. Ezrin is
phosphorylated by various protein-tyrosine kinases and modulates interactions between cytoskeletal and membrane proteins.
Annexin A3 has a role in DNA synthesis. Acidic ribosomal phosphoprotein P0 and Grp75 play in protein synthesis. These results
suggest that the proteins related to the MAP kinase cascade had some role in continuous proliferation of HSL cells without
differentiation. |
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Keywords: | Proteomics Hepatic stem-like cells Two-dimensional electrophoresis Butyrate Differentiation |
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