新生大鼠脊髓交感节前神经元的单胺能受体

应用新生大鼠离体脊髓薄片的细胞内记录，观察了５－羟色胺（５－ＨＴ）、去甲肾上腺素（ＮＡ）及肾上腺素（ＡＤ）对交感节前神经元（ＳＰＮ）膜电位的作用，以期阐明三种单胺受体是共存于同一细胞，还是分别存在于不同细胞？实验结果显示：（１）５－ＨＴ，ＮＡ及ＡＤ分别使５７．１％（１６／２８），６０％（１５／２５）及５２．４％（１１／２１）的ＳＰＮ出现了去极化反应；（２）根据ＳＰＮ对三种单胺的反应，可将６０％（１

RECEPT0RS 0F MONOAMINE IN SYMPATHETIC PREGANGLIONIC NEURONS OF NE0NATAL RAT SPINAL C0RD IN VITR0

By means of intracellular recordings from spinal cord slices of neonatal rats in vitro,the effects of 5-hydroxytryptamine (5-HT), nor-adrenaline (NA) and adrenaline (AD)on membrane potential in sympathetic preganglionic neurons (SPN) were observed,in order to clarify whether these neuron contain a single type of the monoamine receptor or in cornbination with more than one type of receptors. The results showed that: (1) 5-HT,NA and AD induced membrane depolarization respectively in 57 .1l % (16/28 ), 60%(15/25) and 52.4% (11/21 ) of SPN. (2) According to the reactions of SPN to the three monoamines, several subtypes of SPN could be divided: those sensitive to all the three monoarnines (3/l9), those sensitive to two of them (9/19), those only sensitive to one trpe of monoandnes (4/19) and those insensitive at all (3/19). The significance of coexistence of more than one type of the three monoamines in a single neuron remains to be elucidated.