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Phylogenetic analysis of a free-living strain of Symbiodinium isolated from Jiaozhou Bay, P.R. China |
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| Authors: | WanLi Gou Jun Sun Yu Zhen ZhiGang Yu RongXiu Li |
| Institution: | a Shanghai Research Center of Biotechnology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, PR China b College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China c College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, PR China |
| Abstract: | This paper reported the isolation and the phylogenetic analysis of a free-living Gymnodinium-like dinoflagellates (G. sp. (15)) from the sea water collected at Jiaozhou bay, Qingdao, Shandong Province, P.R. China. Phylogenetic reconstruction analysis with Neighbor-Joining (NJ) method using sequences of variable regions (V1+V2+V3) of the small subunit (SSU) rDNA indicated that G. sp. (15) was a Symbiodinium sp., which was closely related to a symbiont of anemones, S. californium, and the free-living strain, Gymnodinium varians. These three strains formed a new clade (which had been designated as clade F) with 100% bootstrap support. Sequence comparison showed that sequences of the internal transcribed spacer (ITS) of these three strains were highly homologous, suggesting that they might belong to one species. |
| Keywords: | Free-living Molecular phylogeny Symbiodinium Symbiosis Zooxanthellae |
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